Fig. 7.
Effect of PRLR deficiency on H2O2-induced cell death in mouse astrocytes. a Mouse cortical astrocytes derived from wild-type (Prlr+/+) mice were pre-incubated for 24 h in the absence or presence of increasing concentrations of prolactin (PRL) (0.01–10 nM), then 400 μM hydrogen peroxide (H2O2) or vehicle (Veh) was added and incubated for 3 h. Data are means ± SEM of three independent experiments (n = 3) carried out in triplicate. b Mouse cortical astrocytes derived from wild-type (Prlr+/+) or null (Prlr−/−) mice were pre-incubated for 24 h in the absence or presence 1 nM PRL, then 400 μM H2O2 or vehicle was added and incubated for 3 h. Cell viability was quantified by MTT assay, and the results were normalized to PRLR wild-type control treated with vehicle. Data are means ± SEM of three independent experiments (n = 3) carried out in triplicate. One-way ANOVA followed by Tukey’s test. *p < 0.05, **p < 0.001 versus vehicle or indicated group