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. 2024 Jun 1;14:12618. doi: 10.1038/s41598-024-63041-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Perfusion parameters of recovered porcine VCAs following 48 h preservation. At t = 2 h, the line marks the perfusate switch to whole blood at 37 °C. (a) Flow rate profile during SNMP and NMP recovery showing a significantly higher flow allowed by (b) significantly lower vascular resistance in the SC group during the SNMP phase. No significant difference was found between the three groups in the NMP phase. Note: A mixed-effects model with the Geisser-Greenhouse correction was performed to compare groups during each phase. (c) Weight gain following SNMP and NMP was significantly lower in the SC group compared to CS + SNMP GROUP but not with CS GROUP. (d) Aspect of the stored VCA following 48 h supercooling, showing the absence of ice nucleation.