Fig. 8. Early-stage apoE4-Aβ coaggregates from the brains of APOE4/4 AD patients are poorly lipidated.

A Representative two-color TIRF images of early-stage aggregates extracted from frontal cortices of APOE4 homozygotes, before and after immunoprecipitation (IP) with the non-lipidated-apoE4-specific antibody, HAE-4. B Sizes of individual Aβ-containing species before (number of aggregates: 3814, average size: 0.28 ± 0.69 µm) and after (number of aggregates: 3576, average size: 0.19 ± 0.26 µm) immunoprecipitation (data are plotted in log₁₀ scale in inset). C, D Effect of immunoprecipitation on colocalization between Aβ and apoE4 quantified by aggregate counting (C), and fluorescence intensity (D). Data points in panels (C, D) represent one of three biological replicates; error bars represent mean values +/− standard deviation of theree independent experiments. Statistical significance was calculated using a paired two-sample t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ns, non-significant (P ≥ 0.05). Source data are provided as a Source Data file.