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. 2024 Jun 2;14:12654. doi: 10.1038/s41598-024-62544-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Proliferation kinetics. A(a, b)—Total cell yield of individuals and pools; A( c, d)—Colony forming unit-fibroblast (CFU-F) of individual and pooled samples; B(a, b, c)—Population doubling time of Pools 1, 2, and 3 compared to their individual donors; C(a, b, c)—Cumulative population doubling of pools 1, 2, and 3 compared to their individual donors. Marked heterogeneity observed in terms of cell yield, PDT, and CFU-F ability among the individual samples. The % CVs for pools are comparatively lower than that for the individuals for the proliferation kinetics; D(a, b)—Representative image of CFU-F colonies from 100 seeded cells of individual and pooled MSCs respectively.