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Schematic of RNA enrichment and depletion strategies tested in this study. (A) Total RNA sequencing. (B) Poly(A) enrichment with magnetic d(T) beads. (C) Terminator 5′ Phosphate Dependent Exonuclease treatment. (D) RNase H-based ribosomal depletion using sequence specific DNA probes. RNA species are color-coded with rRNA in dark blue, mRNA in teal, and ncRNA in yellow. The Terminator exonuclease is shown in purple, RNase H in green and DNA probes in red.
