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Impact of tissue microenvironment on EV‐mediated cancer‐noncancerous cell communication. Possible effects are: (1) Altered cancer cell properties affect EV components and characteristics. (2) Physical barriers, attachment to cell surface and ECM, and interstitial flow. (3) Preferences in cell types that take up EVs. Selecting optimal physiological models that retain/mimic tissue environment is required to address these questions.
