Skip to main content
. Author manuscript; available in PMC: 2024 Jun 3.
Published in final edited form as: Mol Imaging Biol. 2018 Apr;20(2):213–220. doi: 10.1007/s11307-017-1107-5

Figure 2.

Figure 2.

Comparing NIR sensitivity of a dedicated NIR detector and an add-on detector to pre-existing microscope. Above) Serial dilutions of ICG ranging from 0.47μg/L - 1007μg/L. The same plates were imaged using the Leica platform and the VisionSense platform. Below each well is the signal to background ratio of the corresponding well in the respective visualization systems. An SBR = 1 shows that the fluorescence signal is the same as background == black. Below) Plot of SBR vs log concentration ranging from 1.30μM – 0.6nM. The SBR peaks near 3 for System 1 and 12 for System 2. At concentrations above 80nM, there is auto-quenching of the fluorophores.