| Steps | Temperature | Time | Cycles |
|---|---|---|---|
| Initial Denaturation | 98°C | 2 min | 1 |
| Denaturation | 98°C | 10 s | 20 cycles |
| Annealing | 62°C (−0.5°C /cycle) | 5 s | |
| Extension | 72°C | 1 s per 100 bp | |
| Denaturation | 98°C | 10 s | 10 cycles |
| Annealing | 50°C | 5 s | |
| Extension | 72°C | 1 s per 100 bp | |
| Final extension | 72°C | 5 min | 1 |
| Hold | 4°C | forever | |
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