FIG. 3.

trans-Activation of representative EHV-1 IE, early, γ1 late, and γ2 late promoters linked to the CAT reporter gene by effector constructs expressing the EICP22 and/or EICP0 proteins. L-M cells were transfected with each promoter-CAT reporter construct and 0.3 pmol of either pCDR4 (EICP22 expression construct) and/or pSVICP0K. Transfected cells were harvested, and CAT assays were performed as described previously (2). Each transfection was performed in triplicate, and the experiment was carried out independently three times. Error bars, standard deviations. (A) trans-Activation of the EHV-1 IE reporter construct (pIE-CAT; 1.4 pmol); (B) trans-activation of the EHV-1 early (E) TK reporter construct (TK2-CAT; 1.4 pmol); (C) trans-activation of the EHV-1 γ1 late IR5 promoter (pIR5-CAT; 1.4 pmol); (D) trans-activation of the EHV-1 γ2 late gK promoter (pgK-CAT; 2.0 pmol).