FIG. 6.

Relative abilities of EICP0 deletion mutants d105, ΔACID, ΔNLS, ΔSRICH, ΔRING19-39, and ΔRING8-46 to trans-activate representative EHV-1 promoters. The construction of each mutant was described in Materials and Methods. L-M cells were transfected with each promoter-CAT reporter construct (1.4 pmol of pIE-CAT, pTK2-CAT, or pIR5-CAT; 2.0 pmol of pgK-CAT) and 0.3 pmol of each effector construct. Transfected cells were harvested, and CAT assays were performed as described previously (2). Each transfection was performed in triplicate in individual experiments. Each experiment was carried out independently at least three times. Values obtained for the mutants are expressed as percentages of the wild-type value, which was set at 100%.