TABLE 1.

Comparison of in vitro human cell culture models: patient derived cell cultures and characterized cell lines.

	Patient derived cell cultures	Characterized cell lines
Patient related strategy	Establishment of cell cultures for each individual patient	Cell lines with candidate variants applicable to multiple patients
Informed consent from patient	Cell culture establishment and maintenance	Genetic screening (identification of epilepsy related variant)
Cell model genetic background	Patient specific	Establish and well characterized
Cell model system development	Isolation of patient derived cells	Genetic manipulations of cell lines
Introduction of gene variants	No	Particular epilepsy related variants need to be introduced
Control samples	Isogenic cell line or non affected tissue	Parental cell line