FIG. 3.
Representative example of an HTA. A radioactively labeled single-stranded probe was prepared from HIV-puroBCSG3 plasmid DNA by asymmetric PCR using the segment 9 primer pair (lane P). Segment 9 is 819 bp long. The sequence difference between the two strains within this segment is 4.4%. The probe was annealed with PCR products amplified from HIV-puroBCSG3 plasmid DNA (lane S, homoduplex control), HIV-gptHXB2 plasmid DNA (lanes H, heteroduplex controls), and genomic DNAs from different target cell clones (lanes 1 to 15). The plus sign indicates a band shifted due to heteroduplex formation. The minus sign indicates a nonshifted homoduplex band. Lanes 3, 7, 9, 16, and 18 are shifted compared to the band for HIV-puroBCSG3 DNA and were therefore scored as recombinants.