FIG. 2.

Deletion analysis of HBV EnII. The deletion mutants are shown at the left. Open boxes represent the EnII sequences, and the numerals indicate nucleotides of the HBV genome. The fragment containing the EnII region was cloned into pTATALUC just upstream of the E1b minimal promoter in the antisense orientation. Plasmid DNA was transfected into HepG2 cells, and luciferase activity was determined for the lysate of each sample. Fold stimulation of luciferase activity (mean ± standard deviation) is shown on the right.