FIG. 8.

Transcriptional regulation of nt 1640 to 1663 by HLF, FTF, and E4BP4 in HuH7 cells (A) and HepG2 cells (B). pTATALUC, pTATALUC-E5, which contains five iterations of nt 1640 to 1663 in tandem, pTATALUC-EII2, which contains HBV nt 1640 to 1771, or pTATALUC-EII3, which contains HBV nt 1660 to 1771, was cotransfected with pFLAG-CMV-2, pCMVHLF, pCMVFTF, or pCMVE4BP4, and the cell lysate was assayed for luciferase activity. The fold activity of each transfectant relative to the cells transfected with pTATALUC and pFLAG-CMV-2 was calculated.