IP buffer	20 mM Tris, pH 7.5
	150 mM NaCl
	2 mM EDTA
	1% (v/v) NP40
	0.5 mM DTT (added fresh)
RIPA buffer	10 mM Tris-HCl (pH 8.0)
	1 mM EDTA
	0.5 mM EGTA
	1% Triton X-100
	0.1% sodium deoxycholate
	0.1% SDS
	140 mM NaCl
High-salt wash buffer	IP buffer with 500 mM NaCl
Dephosphorylation buffer or 1× NEB Buffer 3	50 mM Tris-HCl, pH 7.9
	100 mM NaCl
	10 mM MgCl2
	1 mM DTT
Polynucleotide kinase (PNK) buffer without DTT	50 mM Tris-HCl, pH 7.5
	50 mM NaCl
	10 mM MgCl2
Polynucleotide kinase (PNK) buffer with DTT	70 mM Tris-HCl, pH 7.6
	10 mM MgCl2
or 1 × NEB PNK buffer	5 mM DTT
Acid phenol/chloroform, pH 4.5	Ambion, AM9720
2× Proteinase K buffer	100 mM Tris-HCl, pH 7.5
	150 mM NaCl
	12.5 mM EDTA
	2% (w/v) SDS
10 × RNA ligase buffer without ATP or 10× NEB RNA ligase buffer	500 mM Tris-HCl, pH 7.6,
	100 mM MgCl2
	10 mM DTT
10× RNA ligase buffer with ATP or 10× Thermo scientific RNA ligase buffer	500 mM Tris-HCl, pH 7.6
	100 mM MgCl2
	100 mM DTT
	10 mM ATP
Formamide gel loading dye	50 mM EDTA 0.05% (w/v) bromophenol blue formamide ad 100%
Transfer buffer	25 mM Tris 190 mM Glycine 10% methanol
10 × dNTP	2 mM dATP
	2 mM dCTP
	2 mM dGTP
	2 mM dTTP