TABLE 1.
Virion morphology in Malawi Lil-20/1- and Δ9GL-infected macrophage cell cultures and purified virus preparations
| Virus | Cell culture
|
Purified virusa
|
||||
|---|---|---|---|---|---|---|
| No. of infected cells | No. with ACN/total no. (%) | No. with CN/total no. (%) | Titer (log10 TCID50/ml) | No. with ACN/total no. (%) | No. with CN/total no. (%) | |
| Expt 1 | ||||||
| MAL | 121/388 (31) [B = 0/111 (0)]b | 267/388 (69) [B = 111/111 (100)] | ||||
| Δ9GL | 395/416 (95) [B = 60/67 (90)] | 21/416 (5) [B = 7/67 (10)] | ||||
| Expt 2 | ||||||
| 9GL-R | 50 | 66/557 (12) | 491/557 (88) | 9.3 | 59/1,350 (4) | 1,291/1,350 (96) |
| Δ9GL | 50 | 163/175 (93) | 12/175 (7) | 7.3 | 110/137 (80) | 27/137 (20) |
| Expt 3 (Δ9GL) | ||||||
| 24 HPI | 25 | 81/90 (90) | 9/90 (10) | |||
| 48 HPI | 25 | 39/43 (90) | 4/43 (10) | |||
| 72 HPI | 25 | 114/133 (86) | 19/133 (14) | 7.3 | 144/164 (88) | 20/164 (12) |
a
Viruses in 25 random fields were counted and scored.
b
B, particles budding from the cell membrane.