Figure 4.

Transfection with miR-5010-5p mimic changes the PP2A activity and protein expression levels of AMPK and NF-κB. (A) Serine/threonine phosphatase assay was used to determine the activity of PP2A. Western blotting was used to determine the protein expression levels of total and phosphorylated (B) AMPK and (C) NF-κB (p65). HK-2 cells were transfected with 100 pmol of miR-5010-5p mimic and treated with 30 mM of d-glucose. ***p<0.001, **p<0.01, and *p<0.05. AMPK, AMP-activated protein kinase; NF-κB, nuclear factor-kappa B; pAMPK, phosphorylated AMPK; pNF-κB, phosphorylated NF-κB; PP2A, protein phosphatase 2A; HG, high glucose.