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. 2024 May 13;147(6):2053–2068. doi: 10.1093/brain/awae078

Figure 5.

Figure 5

Ectopic expression of NF242 in rNLS8 mice. (A) Kaplan-Meier graph showing the increased lifespan after doxycycline (Dox) retrieval of rNLS8 mice injected with AAV9/GFP (n = 12) compared to mice injected with AAV9/NF242 (n = 11) (P = 0.0195). (B) Representative pictures showing a rNLS8 mouse injected with AAV9/GFP with clasping and a rNLS8 mouse injected with AAV9/NF242 after 5 weeks without Dox. (C) Kaplan-Meier graph showing clasping quantification of rNLS8 mice injected with AAV9/GFP (n = 12) or AAV9/NF242 (n = 12). AAV9/NF242 injected rNLS8 mice show a significant delay in clasping occurrence (P = 0.0075). (D–G) Open field test comparing rNLS8 mice injected with AAV9/GFP (n = 12) or AAV9/NF242 (n = 12). AAV9/NF242 injected rNLS8 mice show an increase in (D) total distance travelled (P = 0.0222), (E) horizontal activity (P = 0.0488), (F) movement time (P = 0.0105) and (G) a decrease in resting time (P = 0.0105). (H) Representative visualizations of gait assessment (Catwalk) that compares the improved gait pattern of an AAV9/NF242 injected rNLS8 mouse with an AAV9/GFP injected rNLS8 mouse at 6 weeks without Dox. Wild-type mouse shows normal gait. (I–K) Catwalk quantification comparing rNLS8 and wild-type (wt) mice injected with AAV9/GFP or AAV9/NF242 (n = 12 for each group of rNLS8 mice; n = 6 for each group of wild-type mice). The AAV9/NF242 injected rNLS8 mice show an improvement in (I) the forelimb maximum area (P = 0.0251), (J) the hindlimb maximum area (P = 0.0211) and (K) the hindlimb swing speed (P = 0.0438). (L) Grip force experiment showing that the AAV9/NF242 injected rNLS8 mice (n = 12) have a slight increase in the force compared to the AAV9/GFP injected mice (n = 12) (P = 0.0441). Wild-type mice injected with AAV9/GFP (n = 6) or AAV9/NF242 (n = 6) are shown as healthy grip force controls. GFP = green fluorescent protein.