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Effects of nuclear 3′ processing on abundance and structure of different intron circles. The distribution of trimmed intron circles for all tested introns has a limit of 15–20 nt of lariat tail remaining, independent of the initial tail length. Two introns with natural BP–3′ss tails of 14 nt (RPL19A) or 17 nt (ARF2) are not trimmed. Detailed data are shown in Supplemental Table S5.
