Figure 6.

Evidence for full-length and processed circles from human cells. (A) Selected reads from major (U2) spliceosomal introns found by remapping the branchpoint enrichment libraries from Mercer et al. (2015) to permuted intron target sequences from the human genome. Junctions between the 5′ and 3′ intron ends are denoted by a forward slash. Splice site nucleotides from the permuted target intron are highlighted in green. Dashes were added to the target sequence to maintain alignment when nongenomic As are present in the read. Nucleotides in the read that match the splice sites are in bold, and nongenomic As are highlighted in pink. Dashes were added to the read sequence to maintain alignment where nucleotides from the intron 3′ end are missing. (B) Selected reads from minor (U12) spliceosomal introns found by remapping the branchpoint enrichment libraries (Mercer et al. 2015) to permuted intron target sequences from the human genome. Highlights and dash placement are the same as for A. Additional examples with read identifiers are shown in Supplemental Table S6.