Figure 3.

Single-cell RNA sequencing (scRNA-seq) of Tau P251L knock-in brains. (A) Schematic of the scRNA-seq analysis pipeline. Following dissection, brains were dissociated in the enzymatic solutions, and the single-cell suspension was encapsulated by the 10x Genomics Chromium platform. The 10x libraries were prepared and sequenced, and after quality control, data were analyzed. (B) UMAP representation of the six integrated scRNA-seq runs: three control and three Tau P251L knock-in. The integrated data set contains 130,489 cells, and 26 clusters out of 29 were annotated. (C) Percentage expression heatmap of the highly expressed marker genes within all clusters. Flies are 10 d old.