FIG. 4.

(A) Details of deletion mutations introduced at the outboard junction of the MVM insert in pR1-4AGA. In constructs 1, 2, 6, and 7, the final nucleotide of viral sequence (as indicated) is linked directly to the first nucleotide of vector sequence, indicated in lightface. In construct 4, MVM nucleotide 5042 is linked directly to the T residue seven nucleotides into the vector sequence in the bottom strand. Constructs 3 and 5 have three nucleotides, 5′-CTT-3′ in the bottom strand, inserted between the viral and vector sequences. (B and C) Autoradiographs of total replication products (B) and anti-NS1 immunoprecipitates of replication products (C) generated using substrates 1 through 7, with each lane numbered according to the substrate used.