FIG. 2.

Autoradiographic images of electrophoretically separated infected cell proteins bound to GST or to the GST–IC-1a fusion protein. HEp-2 cells were mock infected or exposed to 10 PFU of HSV-1(F) per cell in medium 199V and labeled with [³⁵S]methionine between 12 and 16 h after infection. Lysates of infected cells were reacted with GST or GST–IC-1a fusion protein bound to glutathione-agarose beads. After extensive rinsing, the protein complexes bound to beads were subjected to electrophoresis on an SDS–12% polyacrylamide gel, transferred to a nitrocellulose sheet, and subjected to autoradiography. Lanes 1 and 2, whole-cell extracts from mock-infected or HSV-1(F)-infected HEp-2 cells, respectively; lanes 3 and 4, HSV-1(F)-infected cell proteins bound to GST–IC-1a and to GST, respectively. Arrows indicate two prominent bands of infected cell proteins specifically precipitated by GST–IC-1a.