FIG. 3.
Isolation of infectious virus from brains of infected animals. CD46 C-Cyt1 transgenic MCP-10 (triangles), CD46 C-Cyt2 transgenic MCP-7 (squares), and nontransgenic (diamonds) mice were inoculated 2 days after birth with 3 × 105 TCID50 of MV (Edmonston strain). At indicated times, animals were sacrificed, and brains were dissected, weighed, and homogenized. After freezing-thawing, viral titers in homogenates were evaluated on Vero monolayers. Mean log10 (TCID50) per brain ± SD is presented. Two to five mice per group were analyzed. The detection limit of the method used was 2 log10 per brain.