FIG. 5.

Nucleosomal DNA fragmentation in brains of infected animals. Transgenic and nontransgenic (NonTg) mice were inoculated 2 days after birth with 3 × 10⁵ TCID₅₀ of MV (Edmonston strain). At day 5, animals were sacrificed, different brain structures were dissected, and DNA was isolated and analyzed on a 1.5% agarose gel. Nucleosomal DNA fragmentation in the cerebellum (10 μg of low-MW DNA) (A) and hippocampus (5 μg of low-MW DNA) (B) of CD46 Cyt1 transgenic (MCP-10), CD46 Cyt2 transgenic (MCP-7), and nontransgenic MV-infected mice is shown. (C) Nucleosomal fragmentation in the frontal cortex (50 μg of low-MW DNA) of MV- but not MV-UV-inoculated CD46 Cyt2 (MCP-7) mice. Lane M, 200-bp DNA marker. Results are representative of three different experiments.