FIG. 3.

Immunoblot analysis examining oligomer formation of the gB insertion mutants. Transfected cell lysates were resuspended in nonreduced sample buffer, resolved by SDS-PAGE on a 7.5% gel, and electrotransferred to nitrocellulose. The blots were probed with a combination of MAbs 27-78 and 58-15 as described in Materials and Methods. The HCMV lane represents cell lysates from 7-day-infected fibroblast cells; mock lane represents cell lysates from untransfected 293T cells. HMW, high molecular weight.