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Immunoblot analysis examining proteolytic processing of the gB insertion mutants. Transfected cell lysates were resuspended in reduced sample buffer, resolved by SDS-PAGE on a 10% gel, and electrotransferred to nitrocellulose. The blots were probed with a combination of MAbs 27-78 and 58-15 as described in Materials and Methods. The HCMV lane represents cell lysates from 7-day-infected fibroblast cells; the mock lane represents cell lysates from untransfected 293T cells.
