FIG. 6.
Viral proteins in fMHV-infected cells. FCWF cells infected with fMHV and, for comparison, FIPV-infected FCWF cells and MHV-infected LR7 cells were labeled for 1 h with 35S-amino acids. Immunoprecipitations were performed on aliquots of cleared lysates of these cells by using the following antibodies (Ab.): K134 rabbit serum against purified MHV-A59 (αMHV); serum G73 from a FIPV-infected cat (αFIPV); and MAb WA3.10 and 23F4.5, recognizing the ectodomains of MHV S (αSm) and FIPV S (αSf), respectively. As indicated, proteins were heated at 95°C (+) or analyzed without heating (−) in SDS–12.5% polyacrylamide gels. The positions of the S, M, and N proteins in the gel are indicated on the left for MHV and on the right for FIPV.