Table 1. Cryo-EM data collection and processing statistics.
| K125E90 | K125R90 | LMNG90 | K125EHEPES | WTHEPES | |
|---|---|---|---|---|---|
| Voltage (kV) | 300 | 300 | 300 | 300 | 300 |
| Magnification (×1000) | 105 | 105 | 105 | 75 | 75 |
| Camera | K3 | K3 | K3 | Falcon 3 | Falcon 3 |
| Frame alignment on Falcon 3 | – | – | Yes | Yes | |
| Camera mode | Super-resolution | Counting bin 1 | Super-resolution bin 2 | Counting | Counting |
| Energy filter (eV) | 20 | 20 | 20 | 20 | 20 |
| Defocus range (μm) | –0.8 to –2.0 | –0.8 to –2.0 | –0.8 to –2.3 | –0.3 to –1.7 | –0.5 with Volta phase-plate |
| Pixel size (Å/pix) | 0.85 | 0.835 | 0.835 | 1.08 | 1.08 |
| Dose on detector (e–/pix/s) | 10 | 15 | 18.77 | 0.7 | |
| Dose on sample (e–/pix/s) | 11.4 | 18 | 1.06 | 0.69 | |
| Exposure time (s) | 3 | 2 | 2 | 44.01 | 60 |
| No. of images | 4731 | 10,044 | 11,362 | 2573 | 2436 |
| Frames per image | 45 | 50 | 50 | 40 | 75 |
| Final particle number | 189,887 | 222,622 | 204,438 | 147,546 | 60,995 |
| Resolution* | |||||
| Masked D6 (Å) | 2.2 | 2.1 | 2.0 | 4.3 | 4.9 |
*
From Relion_postprocess (Scheres, 2012).