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. 2024 Apr 20;38(6):1236–1245. doi: 10.1038/s41375-024-02222-w

Fig. 3. 8CA/8AA and VEN synergistically target the viability and methylations of AML cell lines.

Fig. 3

A, B MV4-11 and KG-1a cells were treated with vehicle (NT), 10 nM or 20 nM VEN for 48 h, then the cellular methionine levels were measured through mass spectrometry (n = 3). C–E MV4-11 and KG-1a cells were treated with vehicle (NT), 1 μM 8CA/8AA, 0.02 μM VEN or their combinations for 48 h, then the intracellular SAM were measured through mass spectrometry (n = 3) and the histone methylations H3K4me3, H3k9me3, H3K27me3, and H3K36me3 were measured through western blotting. F MV4-11 and KG-1a cells were treated with 8AA, VEN or 8AA plus VEN at a series of concentrations for 48 h, and the cell viability was measured (n = 4). The combination index (CI) is calculated through CompuSyn software to define drug synergism (CI < 1.0), addition (CI = 1.0) and antagonism (CI > 1.0). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.