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. 2024 May 30;25(6):1083–1096. doi: 10.1038/s41590-024-01844-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, Representative flow cytometry plots (left) and quantification (right) of spleen cells from 8- to 10‐week‐old wild-type, 10E8-NGS-04^H and 10E8-UCA^H mice and gating strategy for the quantification of splenic B220⁺TCRβ⁻ B cells and TCRβ⁺B220⁻ T cells. Data are pooled from two independent experiments (n = 3–4 mice per independent group). b, Representative flow cytometry plots (left) and quantification (right) of spleen cells from 8- to 10‐week‐old wild-type, 10E8-NGS-04^H and 10E8-UCA^H mice and gating strategy for the quantification of CD21^hiCD24^lo follicular B cells, transitional CD21^loCD24^hi T0/T1 B cells, CD21^hiCD24^hiCD23⁺ T2 B cells and CD21^hiCD24^hiCD23⁻ marginal zone B cells. Data are pooled from two independent experiments (n = 3–4 mice per independent group). Error bars indicate mean ± s.d. from mice in pooled groups. c, Representative flow cytometry (left) and quantification (right) of B220⁺CD4⁻CD8⁻F4/80⁻Gr-1⁻ naive B cells from peripheral blood binding biotinylated 10E8-GT9 in wild-type or 10E8-NGS-04^H mice. Data are pooled from two independent experiments. Error bars indicate mean ± s.d. from mice in each group (n = 3–6). d, Representative flow cytometry (left) and quantification (right) of naive B cells binding to biotinylated 10E8-GT9 in wild-type or 10E8-UCA^H mice. Error bars indicate mean ± s.d. from mice in each group (n = 3). Data from a single experiment are presented and are representative of three independent experiments. e, Top, 10x Genomics scBCR-seq data from 10E8-GT10.2-specific naive B cells from 10E8-NGS-04^H mice (n = 1,026 pairs amplified) showing human 10E8-NGS-04 IGH-V gene frequency (left) and mouse IGK-V genes (right). Cells from three mice were sequenced, and representative data from one mouse are presented. Bottom, 10x Genomics scBCR-seq data from 10E8-GT10.2-specific naive B cells from 10E8-UCA^H mice (n = 1,418 pairs amplified) showing the frequency of the human 10E8-UCA IGH-V gene (left) and mouse IGK-V genes (right). Cells from three mice were sequenced, and representative data from one mouse are presented; HC, heavy chain; LC, light chain.