Extended Data Fig. 5. NGS-04^H mice show weak immune responses to 10E8-GT10.2, related to Fig. 3.

a, Graph showing mean fluorescence intensity (MFI, % of initial) of 10E8-GT10.2 dissociation from 10E8-UCA^H (green) and 10E8-NGS-04^H BCRs (teal). Error bars indicate SD (n = 4). Experiment performed once. b, Schematic showing wild-type mice adoptively transferred with 10E8-NGS-04^H precursors and immunized with either 50 µg of 10E8-GT9.6 12mer, 10E8-GT10.2 12mer or 10E8-GT9-KO 12mer with ribi adjuvant (control), as in Fig. 3b, c. c, Schematic showing wild-type mice adoptively transferred with 10E8-NGS-04^H precursors and immunized with either 5 μg 10E8-GT10.2 12mer and alhydrogel or 5 μg 10E8-GT9-KO 12mer control and alhydrogel. d, Representative flow cytometric plots of CD45.2⁺10E8-NGS-04^H B cells recruitment to GCs 7 and 14 DPI in spleens of mice immunized as in c. e, Quantification of GC size and CD45.2 in GC in spleen of mice immunized in c,d. Data are pooled from two independent experiments (n = 5–6 per treatment). Error bars indicate mean ± SD from mice in each group.