Extended Data Fig. 7. Antigens with a minimum affinity can recruit 10E8-UCA^H B cells to germinal centers, related to Fig. 3.

a, Schematic showing mice adoptively transferred to reach ~1:10⁴ 10E8-UCA^H precursors and immunized with 5 µg either different variants of 10E8-GT9 12mer and GT10.2 12mer with alhydrogel or 10E8-GT9-KO 12mer with alhydrogel (control); experiment performed once. b, Quantification of GC size, CD45.2⁺10E8-UCA^H B cells in GC and epitope specific CD45.2⁺10E8-UCA^H in GC in spleen of mice immunized as in a. Error bars indicate mean ± SD from mice in each group (n = 5). c, Quantification of GC size, CD45.2 in GC and epitope specific CD45.2 in GC in lymph nodes of mice immunized in a. Error bars indicate mean ± SD from mice in each group (n = 5).