Fig. 1. Distinct platelet replenishment kinetics from single HSCs.

a, HSPC hierarchy reconstituted by single LSKGata1-eGFP⁻CD34⁻CD150⁺CD48⁻ Vwf-tdTomato⁻ multi-HSCs (n = 8) or Vwf-tdTomato⁺ P-HSCs (n = 9). The numbers shown are the mean ± s.e.m. percentage contributions to each population. Orange, reconstitution in all mice. Pink, reconstitution in some mice (the fraction of reconstituted mice is indicated in the upper left of each circle); mean of positive mice. Only progenitor populations present in ≥1/3 of mice and with ≥0.1% average reconstitution are shown. For the full P-HSC hierarchy, see Extended Data Fig. 1d. Phenotypic definitions: LT-HSC, LSKFLT3⁻CD150⁺CD48⁻CD45.2⁺; ST-HSC, LSKFLT3⁻CD150⁻CD48⁻CD45.2⁺; MPP2, LSKFLT3⁻CD150⁺CD48⁺CD45.2⁺; MPP3, LSKFLT3⁻CD150⁻CD48⁺CD45.2⁺; MPP4, LSKFLT3⁺CD45.2⁺; MkP, LKCD150⁺CD41⁺CD45.2⁺; preMegE progenitor, LKCD41⁻CD16/32⁻CD150⁺CD105⁻CD45.2⁺; colony-forming unit-erythroid (CFU-E), LKCD41⁻CD16/32⁻CD150⁻CD105⁺Gata1-eGFP⁺; pregranulocyte–monocyte (preGM) progenitor, LKCD41⁻CD16/32⁻CD150⁻CD105⁻CD45.2⁺; GMP, LKCD41⁻CD16/32⁺CD150⁻CD105⁻CD45.2⁺; platelets (P), CD150⁺CD41⁺TER119⁻Vwf-tdTomato⁺Gata1-eGFP⁺ for Vwf-tdTomato^tg/+ Gata1-eGFP^tg/+ donors; erythrocytes (E), TER119⁺CD150⁻CD41⁻Vwf-tdTomato⁻Gata1-eGFP⁺; myeloid (granulocyte and monocyte) cells (M), CD11b⁺NK1.1⁻CD19⁻CD4/CD8a⁻CD45.1⁻CD45.2⁺; donor-derived B lymphocytes (B), CD19⁺NK1.1⁻CD4/CD8a⁻CD11b⁻CD45.1⁻CD45.2⁺; donor-derived T lymphocytes (T), CD4/CD8a⁺NK1.1⁻CD11b⁻CD19⁻CD45.1⁻CD45.2⁺. b, HSCs and MPPs replenished by Vwf-tdTomato⁻ multi-HSCs (n = 8) or Vwf-tdTomato⁺ P-HSCs (n = 9). Representative profiles and mean ± s.e.m. percentages of the parent LSKCD45.2⁺ gate are shown. c, Granulocyte/monocyte (GM) and megakaryocyte (MK) in vitro lineage potentials (mean ± s.e.m.) of HSCs and MPPs replenished by Vwf-tdTomato⁻ multi-HSCs (n = 3). Data are from 580–720 plated wells per population with a similar distribution across three replicates. Each dot represents an independent experiment.

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