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. 2024 May 30;25(6):1073–1082. doi: 10.1038/s41590-024-01833-w

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© The Author(s) 2024, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a, Representative gating scheme. b, Enrichment of HCDR3 lengths among epitope-specific B cells over unsorted controls as in Fig. 4d. c, Frequency of long (>=20aa) HCDR3s among epitope-specific B cells (sorted) as in b, or among total IgM+ naïve B cells (unsorted). Symbols represent n = 3 (GT9), n = 4 (GT10.1), n = 6 (GT12) or n = 14 (unsorted controls) independent donors. * p = 0.046, **** p < 0.0001, Kruskal-Wallis test with Dunn’s multiple comparison correction. d, Percentage of HCDR3s containing the YxFW motif among epitope-specific B cells as in c. * p = 0.03, *** p = 0.0001, Kruskal-Wallis test with Dunn’s multiple comparison correction. e, Percentage of 10E8-class V_H among epitope-specific BCRs with 10E8-like HCDR3s (with length 21-24 aa and YxFW at correct position within HCDR3) within datasets obtained using the 10x Genomics sequencing method as in c. **p = 0.002, two-sided Mann-Whitney test. f, Percentage of IGVL3 family light chains among 10E8-GT12-sorted BCRs that are either 10E8-class (10E8-class H3) or lack the YxFW motif (non-YxFW). n = 6 independent donors, ns not significant, two-sided Wilcoxon test. g, Percentage of 10E8-GT12-specific naive IgM⁺ BCRs with 10E8-class or LN01-class HCDR3s. Symbols represent n = 6 (GT12) or n = 14 (unsorted controls) independent donors. h, Frequency of 10E8-class or LN01-class B cells among IgM+ naive B cells, detected through 10E8-GT12 sorting as in g. i, SPR-measured monovalent K_d values for 10E8-GT9, 10E8-GT10.2, and 10E8-GT12 monomer binding to 10E8-class and non-10E8-class (competitor) antibodies isolated by the respective scaffolds. Symbols represent different antibodies; lines represent median values.