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. 2024 May 30;25(6):1073–1082. doi: 10.1038/s41590-024-01833-w

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© The Author(s) 2024, corrected publication 2024

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Extended Data Fig. 5 — a, Polyspecificity reagent binding as measured by ELISA. PGT121, VRC01 and PGT128 served as negative controls, MPER bnAb 4E10 as a positive control. NGS-1 through −22 correspond to human NGS precursors described in the main text. These are 10E8-class heavy chain (HC) precursors identified from searching next-generation sequencing (NGS) datasets of primarily naive IgM HCs from 14 HIV-seronegative human donors^5,27 paired with the inferred-germline 10E8 light chain (LC); 10E8-HuGL: bona fide HC/LC pairs isolated by epitope-specific sorting of naïve human B cells (n = 25). b, Mean fluorescence intensity (MFI) of antibodies as in a in a HEp-2 cell autoreactivity assay. c, raw images of data shown in b.