Extended Data Fig. 6. Immunization of MPER-HuGL18^H B cell adoptive transfer recipient mice with 10E8-GT10.2 12mers.

a, Flow cytometry analysis of bone marrow cells from WT (n = 4) and MPER-HuGL18^H (M18, n = 7) mice; gating strategy shown on the left. B-cell progenitors (B220⁺) were divided into immature (CD43⁺) and mature (CD43⁻) cells. Early (CD43⁺) B-cell progenitors were subdivided into Hardy populations A (CD24⁻BP-1⁻), B (CD24⁺BP-1⁻), and C (CD24⁺BP1⁺). Late (CD43⁻) B-cell progenitors were subdivided into Hardy populations D (IgM⁻IgD⁻), E (IgM⁺IgD^int), and F (IgM⁺IgD+). Right bars represent quantifications of these populations, error bars indicate SD. b, Frequency of CD45.2⁺ B cells among splenic B cells, one day after adoptive transfer of 200,000 CD45.2⁺ MPER-HuGL18^H B cells into CD45.1⁺ WT mice. Symbols represent individual animals, error bars indicate SD. c, Germinal center (GC) response to immunization in CD45.1⁺ WT mice adoptively transferred with 2 × 10⁵ CD45.2⁺ MPER-HuGL18^H B cells on Day 21 after immunization with 10E8-GT10.2 12mer or negative control 10E8-GT9-KO 12mer (KO). Left column shows the frequency of total GC (CD38^loCD95⁺) among B cells gated from SSL; right column shows the frequency of CD45.2 B cells among total GC.