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. 2024 May 30;25(6):1073–1082. doi: 10.1038/s41590-024-01833-w

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© The Author(s) 2024, corrected publication 2024

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Extended Data Fig. 7 — a, Illustration of genetic modifications in hD3-3/J_H6 mice (not drawn to scale) with hD3-3 and hJ_H6 segments replacing mouse DQ52 and J_H1-4 segments. Sequences of hD3-3 (in red), J_H6 (in blue) and flanking regions are shown below the diagram. b, Characterization of B220⁺ B cell and CD3⁺ T cell populations among lymphocyte/live cell/single cells from homozygous D3-3/J6 mouse spleens by flow cytometry compared to a wild-type 129SVE mouse (WT). c, Characterization of IgM⁺IgD^hi naïve B cells among B cells as in b. d, Characterization of CD23^hiCD21^low follicular (FO) B cells and CD23^lowCD21^hi marginal zone (MZ) B cells among lymphocyte/live cell/single cell/B220⁺CD93^low mature B cells, as in b. e, Characterization of Igk⁺ and Igl⁺ B cells among B cells as in b. f, Fraction of productive IgH rearrangements that contain hD3-3 or mouse D (mD), based on repertoire analysis of three homozygous hD3-3/J_H6 mice. The hD3-3 usage value represents average ± standard deviation. Since hJ_H6 is the sole J_H segment in homozygous hD3-3/J_H6 mice, all V(D)J recombination events contain hJ_H6. Therefore, 16.3% productive rearrangements in homozygous hD3-3/J_H6 mice contain both hD3-3 and hJ_H6. g, HCDR3 length distribution of productive IgH rearrangements that contain hD3-3 and J_H6 as in f. Error bars indicate SD of three independent mice.