Fig. 5. 10E8-class B cells function in vivo.

a, Flow cytometry analysis and quantification of B220⁺TCRβ^– B cells, B220^–TCRβ⁺ total T cells, CD4⁺CD8⁻ T cells, CD4⁻CD8⁺ T cells, CD2⁻CD24^hi T0/T1 B cells, CD21^loCD24^lo follicular B cells, CD21^hiCD24^hiCD23⁻ T2 B cells and CD21^hiCD24^hiCD23⁺ marginal zone B (MZB) cells in the spleens of MPER-HuGL18^H mice (n = 7) compared to wild-type (WT) C57BL/6 mice (n = 4). Symbols represent individual animals, and error bars indicate standard deviation. b, Frequency of CD38^loCD95⁺ GC B cells among total B220⁺ B cells (left) and CD45.2⁺ B cells among CD38^loCD95⁺ GC B cells (right) at day 21 after immunization with 10E8-GT10.2 12mer (n = 14) or control 10E8-GT9-KO 12mer (n = 8) in CD45.1 wild-type recipient mice adoptively transferred with 200,000 CD45.2 MPER-HuGL18^H B cells. Symbols represent individual animals; bars indicate mean ± s.d.; ****P < 0.0001. Data were analyzed by two-sided Mann–Whitney test. c, Frequencies of 10E8-GT10⁺⁺ cells among CD38^loCD95⁺CD45.1⁻CD45.2⁺ MPER-HuGL18^H GC cells as in b.