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. 2024 May 30;25(6):1073–1082. doi: 10.1038/s41590-024-01833-w

Fig. 6. mRNA-LNP delivery of 10E8-GT12 nanoparticles primes diverse 10E8-class B cells.

Fig. 6

a, Percentage of 10E8-GT10.1++10E8-GT10.1-KO– (epitope-specific) CD19+IgD+ naive B cells with 10E8-class HCDR3s for humans (as in Fig. 4f) and hD3-3/JH6 mice. b, Percentage of 10E8-GT9–KO++10E8-GT9–, 10E8-GT10.1++10E8-GT10.1-KO– or 10E8-GT12++10E8-GT12-KO– epitope-specific IgG+ BCRs with 10E8-class HCDR3s from day 42 after immunization of hD3-3/JH6 mice with 10E8-GT9-KO 12mer (n = 3), 10E8-GT10.2 12mer (n = 12), 10E8-GT12 12mer (n = 5) or 10E8-GT12 24mer (n = 12) delivered as protein in SMNP, respectively, or 10E8-GT12 24mer delivered by mRNA (n = 11). Symbols represent individual animals, and bars indicate median values. c, Percentage of epitope-specific IgG+ BCRs as in b with 10E8-class HCDR3s and at least one proline in position +7 or +8 relative to the YxFW motif from hD3-3/JH6 mice with >100 sequences. Sequences of genes encoding DH in mature 10E8 and iGL are shown with the YxFW motif in green, and the targeted prolines are colored red; **P = 0.006. Data were analyzed by two-sided Mann–Whitney test.