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. 2024 May 30;25(6):1073–1082. doi: 10.1038/s41590-024-01833-w

Fig. 8. 10E8-class BCRs induced by 10E8-GT nanoparticles bind epitope scaffold 10E8-B1 containing a near-native 10E8 peptide epitope.

Fig. 8

a, SPR-measured monovalent Kd values for 10E8-B1 binding to different antibodies containing the indicated number of 10E8-class mutations, including the 10E8 UCA, 10E8-class human naive precursors isolated by human B cell sorting (human naive), artificial partially mature 10E8-class antibodies (intermediates) and mature 10E8. Each symbol represents an antibody; overlapping data are staggered along the x axis. b, Crystal structure of 10E8-B1 in complex with 10E8 bnAb (right), with the structure of 10E8 bound to peptide12 (left) shown for reference. Colors are as in Fig. 3. c, SPR-measured monovalent Kd values for 10E8-B1 binding to iGL antibodies or antibodies recovered after immunization of hD3-3/JH6 mice or macaques with 10E8-GT nanoparticles (after priming). Each symbol represents a different antibody; **P = 0.004. Data were analyzed by a Kruskal–Wallis test with a Dunn’s multiple comparison correction.