FIG. 2.

Immunofluorescence analysis of the SG complex in the TA muscle. (a) Cryosections of the TA muscle from the wild-type (WT) F1B hamster (top row), Bio14.6 hamster treated with the recombinant AAV vector (middle row), and untreated Bio14.6 hamster (bottom row) were immunofluorescently stained with antibodies against the four components of the SG complex (α-SG, β-SG, γ-SG, and δ-SG). Note that the anti-δ-SG monoclonal antibody is specific to the human δ-SG protein. Therefore, it was unable to detect the δ-SG in the wild-type hamster, but it was able to detect the human δ-SG expressed in the recombinant AAV vector-treated Bio14.6 hamster muscle. (b) Cryosections of the TA muscle from another Bio14.6 hamster treated with the recombinant AAV vector. The consecutive sections were immunofluorescently stained with antibodies either against δ-SG (A) or against α-SG (B). Note that overexpression of δ-SG in different regions of the vector-treated muscle did not interfere with the distribution of α-SG on the myofiber sarcolemma.