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. 2024 Mar 27;48(3):1727–1740. doi: 10.1007/s11259-024-10358-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Phylogenetic tree based on partial heat shock gene (groEL) nucleotide sequences of Anaplasma phagocytophilum, A. platys and Ehrlichia canis. Phylogeny was evaluated using the Maximum Likelihood method and the Tamura 3-parameter model implemented on MEGA 11 software version 11.0.11 on multiple alignment constructed with nucleotide sequences obtained in this study, 55 reference sequences of A. phagocytophilum, 17 of A. platys, 8 of A. platys-like and 9 of E. canis retrieved from GenBank. On the left a traditional rectangular branch style of the tree and on the right a radiation branch style of the tree. Identification of the sequences undergoes the following nomenclature: GenBank accession number, species, strain (only for sequences obtained in this study), host, country (AR: Argentina, AU: Australia, BR: Brazil, CL: Chile, CN: China, CU: Cuba, FR: France, IT: Italy, JP: Japan, KN: Saint Kitts and Navis, PH: Philippines, SN: Senegal, TH: Thailand, TN: Tunisia, UR: Uruguay), and collection date (or date of database submission). Statistical support was provided by bootstrapping with 1000 replicates and values indicated on the respective branches. Highlighted in black or black circles: sequences generated in this study. Main clusters of A. phagocytophilum are labelled following the ecotype classification proposed by Jahfari and colleagues (Jahfari et al. 2014)