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. 2024 Jun 3;15:4728. doi: 10.1038/s41467-024-48735-x

Fig. 1. Recombineering of llama IgH BAC transgenes.

Fig. 1

a Two bacterial artificial chromosomes (BAC V03, BAC F07) covering the core of the llama IgH locus were cloned from a liver genomic DNA library and fully sequenced. b BAC recombineering was used to insert five additional VHH-genes, to delete the CH1 exon of IgM, to replace the IgD pseudogene with the IgG2b gene, and to insert elements of the mouse 3’ locus control region (mLCR, αE), resulting in BAC TE02. c BAC recombineering was used to exchange the exons encoding the CH3, CH4, transmembrane, and cytosolic domains of IgM with corresponding murine sequences, resulting in BAC TE03. d Explanation of symbols used in the schematics.