Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Apr 10;43(11):2166–2197. doi: 10.1038/s44318-024-00093-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

PMC Copyright notice

(A) CENP-A is mislocalized in DNAJC9-depleted HeLa YFP-CENP-A^Low cells. Representative images of metaphase chromosome spreads immunostained for CENP-A in siNeg or siDNAJC9.3-transfected HeLa YFP-CENP-A^Low cells. Scale bar: 15 µm. Scale bar for insets: 1 µm. (B) Scatter plots showing CENP-A intensities corrected for background at the centromeric or noncentromeric regions as described in (A), from three biological replicates. Median values are shown above graph. Each circle represents the value from an individual chromosome. Chrs refers to the total number of chromosomes measured per condition from three biological replicates. The mean with standard deviation is shown across all measurements from three biological replicates. P values were calculated using Mann–Whitney U test. (C) CENP-C is mislocalized in DNAJC9-depleted HeLa YFP-CENP-A^Low cells. Representative images of metaphase chromosome spread immunostained for CENP-C and CENP-A in siNeg or siDNAJC9.3-transfected cells. Scale bar: 15 µm. Scale bar for insets: 1 µm. (D) Scatter plots showing CENP-C intensities corrected for background at the centromeric or noncentromeric regions as described in (C), from three biological replicates. Median values are shown above graph. Each circle represents the value from an individual chromosome. Chrs refers to the total number of chromosomes measured per condition from three biological replicates. Mean with standard deviation is shown across all measurements from three biological replicates. P values were calculated using Mann–Whitney U test. (E) NUF2 levels are reduced at the kinetochores in DNAJC9-depleted HeLa YFP-CENP-A^Low cells. Top panel: Representative images of fixed cell immunofluorescence images showing the localization of NUF2 and CENP-A in metaphase cells transfected with siNeg or siDNAJC9.3. Scale bar: 5 µm. Bottom panel: Scatter plots showing NUF2 intensities at the kinetochore corrected for background. Indicated number of cells were analyzed from three biological replicates and each circle represents the value from an individual kinetochore. KTs refers to the total number of kinetochores measured per condition from three biological replicates. Mean with standard deviation is shown across all measurements. P values were calculated using Mann–Whitney U test. (F, G) Increased incidence of CIN phenotypes in HeLa YFP-CENP-A^Low cells. Representative IF images showing micronuclei (F) and defects in chromosome segregation (G) indicated by white arrowheads in DNAJC9-depleted HeLa YFP-CENP-A^Low cells. Scale bar: 15 µm. Bar graphs show percent cells with micronuclei (F) and defective chromosome segregation (G) in cells transfected with control (siNeg) or siDNAJC9.3. Mean with SD was plotted from three biological replicates and P value was calculated using unpaired t test. N denotes the total number of cells analyzed per condition. Source data are available online for this figure.