Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Apr 10;43(11):2166–2197. doi: 10.1038/s44318-024-00093-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

PMC Copyright notice

(A) Transcript levels of CENP-A are not altered in DNAJC9-depleted HeLa YFP-CENP-A^Low cells. Bar graphs showing mean RNA levels of CENP-A and DNAJC9 normalized to GAPDH in control and DNAJC9-depleted cells using RT-qPCR. Mean values with standard deviation from three biological replicates were plotted and P values were calculated from two-way ANOVA with Sidak’s multiple correction test. (B) Stability of YFP-CENP-A is higher in DNAJC9-depleted HeLa YFP-CENP-A^Low cells. Western blot of whole-cell extracts showing DNAJC9 depletion and YFP-CENP-A levels in control or DNAJC9-depleted cells treated with 100 µg/ml cycloheximide for the indicated time periods. Alpha-tubulin was used as the loading control. (C) Line graph for results shown in B. Mean with SD from three biological replicates were plotted. (D) Enrichment of CENP-A in chromatin fractions from DNAJC9-depleted HeLa YFP-CENP-A^Low cells. Western blot of soluble and chromatin fractions prepared from control or DNAJC9-depleted cells showing levels of YFP-CENP-A and endogenous CENP-A on chromatin in HeLa YFP-CENP-A^Low. Alpha-tubulin and H2B were used as markers of soluble and chromatin fractions, respectively. (E) Bar graphs showing the levels of YFP-CENP-A or endogenous CENP-A on the chromatin normalized to H2B control in the indicated conditions. Mean with SD were plotted from three biological replicates. P values were calculated using unpaired t test. Source data are available online for this figure.