Reagents and tools table
| Reagent/resource | Reference or source | Catalog number |
|---|---|---|
| Experimental models | ||
| HEK293T cells (H. sapiens) | Aleš Holfeld | N/A |
| HEK293S GnTI- cell (H. sapiens) | Dina Schuster | CVCL_A785 |
| SFC856-03-04 human induced pluripotent stem cells (iPSCs) (H. sapiens) | Haenseler et al, 2017b | N/A |
| SFC856-03-04 SNCA-/- 12E knockout iPSCs (H. sapiens) | Haenseler et al, 2017b | N/A |
| Antibodies | ||
| Motavizumab | Sesterhenn et al, 2020 | N/A |
| D25 | Sesterhenn et al, 2020 | N/A |
| 5C4 | Sesterhenn et al, 2020 | N/A |
| Palivizumab | Sesterhenn et al, 2020 | N/A |
| 101 F | Sesterhenn et al, 2020 | N/A |
| InVivoMAb human IgG1 isotype control | Bio X Cell | #BE0297 |
| Rabbit anti-Alpha-synuclein antibody (MJFR1) | Abcam | 138501 |
| Recombinant proteins | ||
| Respiratory syncytial virus fusion (RSVF) glycoprotein | Sesterhenn et al, 2020 | N/A |
| Calmodulin | Merck | P1431 |
| Ras-related protein Rab5A (GTP- and GDP-bound form) | Alison K Gillingham | N/A |
| Ras-related protein Rab2A (GTP- and GDP-bound form) | Alison K Gillingham | N/A |
| Alpha-synuclein monomer | Dhiman Ghosh | N/A |
| Alpha-synuclein amyloid fibrils | Patterson et al, 2019 | N/A |
| Chemicals, enzymes and other reagents | ||
| Lysyl endopeptidase (Lys-C) | IGZ Instruments | 129-02541 |
| Trypsin | Promega | V5113 |
| TCEP (tris(2-carboxyethyl)phosphine hydrochloride) | Pierce | 20490 |
| Iodoacetamide | Sigma-Aldrich | I1149 |
| Ammonium bicarbonate | Sigma-Aldrich | 9830 |
| Formic acid (FA) | Sigma-Aldrich/Merck | 64‐18‐6 |
| HEPES (4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N¢-(2-ethanesulfonic acid) | Sigma-Aldrich | H4034 |
| Sodium deoxycholate | Sigma-Aldrich | D6750 |
| Proteinase K (PK) from engyodontium album | Sigma-Aldrich | P2308 |
| HRM calibration kit | Biognosys AG | Ki-3002-2 |
| Magnesium chloride hexahydrate | Fluka | 63072 |
| Acetonitrile (ACN), LC-MS grade | ROTISOLV | AE70.1 |
| Potassium chloride | Merck | K41042236-032 |
| Thioflavin T (ThT) | Sigma-Aldrich | T3516 |
| Roche cOmplete EDTA free inhibitor cocktail | Roche | 11873580001 |
| DNAse I | Merck | 10104159001 |
| Software | ||
| Spectronaut 15 | https://biognosys.com/ | N/A |
| SpectroMine 4 | https://biognosys.com/ | N/A |
| R (4.2.2) | https://www.r-project.org/ | N/A |
| Rstudio (2022.07.2) | https://posit.co/download/rstudio‐desktop/ | N/A |
| STRING (version 12.0) | https://string-db.org/ | N/A |
| AlphaFold2 | Varadi et al, 2022; Jumper et al, 2021 | N/A |
| UCSF ChimeraX (1.3rc202111292147) | https://www.rbvi.ucsf.edu/chimerax/ | N/A |
| Other | ||
| BCA protein assay | Pierce | 23228 |
| NativePAGE™ Sample Prep Kit | Invitrogen | BN2008 |
| NativePAGE™ 4 to 16%, Bis-Tris, 1.0 mm, Mini Protein Gels, 10-well | Invitrogen | BN1002BOX |
| NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels) | Invitrogen | NP0060 |
| NuPAGE™ 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well | Invitrogen | NP0321BOX |
| C18 MACROspin plate | The Nest Group | SMM SS18V |
| CLARIOstar Plus plate reader | BMG Labtech | N/A |
| ThermoMixer | Eppendorf | 460‐0223 |
| Column oven | Sonation lab solutions | PRSO‐V2 |
| Orbitrap Eclipse™ Tribrid™ Mass Spectrometer | Thermo Scientific | FSN04-10000 |
| Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer | Thermo Scientific | FETD2-10001 |
| Orbitrap Exploris™ 480 Mass Spectrometer | Thermo Scientific | BRE725533 |
| EASY-nLC™ 1200 | Thermo Scientific | LC140 |
| Waters nanoACQUITY | Waters Corporation | 176016000 |
| HT7700 TEM | Hitachi | TEM HT7700 |