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. 2024 Apr 5;43(11):2127–2165. doi: 10.1038/s44318-024-00084-7

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© The Author(s) 2024, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV4 — (A) Extracellular measurements of 3T3-L1 medium glucose and lactate 24 or 48 h after medium volume change in 12-well plates (n = 3 technical replicates from n = 3 biological replicates). (B) 2-deoxyglucose (DG) uptake after insulin stimulation and 200 µM indinavir (GLUT4 inhibitor) treatment. Cells were cultured in high or low medium for 48 h in 24-well plates prior to the experiment (n = 6 biological replicates). (C) Percentage inhibition of 2-DG uptake after indinavir treatment, calculated from the difference between +/− indinavir treated conditions, as a percentage of -indinavir 2-DG uptake upon 100 nM insulin stimulation. Graph shows the percentage of 2-DG uptake that is GLUT4-dependent (i.e. inhibited by indinavir) (n = 6 biological replicates). (D) Western blot of GLUT1 and GLUT4 in 3T3-L1s after 48 h medium volume change (12-well plate) (n = 6 biological replicates). (E) The pericellular oxygen concentration of primary scAdips cultured with different medium volumes in 96-well plates (n = 4 biological replicates). AA antimycin A. (F) OCR of primary scAdips cultured with different medium volumes in 96-well plates (n = 4 biological replicates). Data information: Data were represented as mean ± SEM (A–D). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by two-way ANOVA with Šidák correction for multiple comparisons (A. B), or by paired two-tailed Student’s t-test (C).