FIG. 5.

VP26-GFP colocalizes with maturing capsids but not accumulated procapsids. COS cells were transfected to express VP26-GFP and then infected with tsProt.A and incubated for 7 h at 39°C (A to F) or 7 h at 39°C followed by 2 h at 31°C (G to L). Fixed cells were immunostained with anti-VP5 monoclonal antibody 6F10 and a Texas red-conjugated anti-mouse secondary antibody and then viewed in the Texas red (B, E, H, and K) or FITC (A, D, G, and J) channel of a laser scanning confocal microscope. Panels C, F, I, and L represent merged images. All scale bars represent 10 μm.