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. 2024 May 21;15:1397579. doi: 10.3389/fimmu.2024.1397579

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Copyright © 2024 Davies, Biryukov, Rill, Klimko, Hunter, Dankmeyer, Miller, Shoe, Mlynek, Talyansky, Toothman, Qiu, Bozue and Cote

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ELISpot assays for IgG-secreting splenocytes from mice given Δcaf1 and ΔyopD/Δcaf1 vaccine regimens, after ex vivo re-stimulation with Y. pestis antigens. Four weeks post-boost, female (red) and male (blue) mice were euthanized and splenocytes re-stimulated with B-Poly S Polyclonal B cell Stimulator for 5 days, then 8 h with rF1-V (A, E), rV (B, F), or temperature-shifted whole-cell CO92 (C, G) and C12 (D, H) antigens, followed by quantification of spots representing cells secreting soluble IgG1 and IgG2a. Splenocytes from mice given Δcaf1 vaccine regimens are shown in (A–D); splenocytes from mice given ΔyopD/Δcaf1 vaccine regimens are shown in E-H. Graphs show each data point and lines representing geometric means. **p < 0.01, ***p < 0.001 in Mann-Whitney test.